Epitope identification from fixed-complexity random-sequence peptide microarrays
– Molecular & Cellular Proteomics, 2014
Antibodies play an important role in modern science and medicine. They are essential in many biological assays, and have emerged as an important class of therapeutics. Unfortunately, current methods for mapping antibody epitopes require costly synthesis or enrichment steps, and no low cost universal platform exists. In order to address this, we tested a random sequence peptide microarray consisting of over 330,000 unique peptides sequences sampling 83% of all possible tetramers and 27% of pentamers.